Evaluation of Neuropharmacological Activities of Different Fractions of Annona reticulata Linn. Leaves

Annona reticulata Linn. was investigated for sedative, anxiolytic, and antidepressant properties. Tail suspension and forced swimming experiments were utilized for assessing antidepressant potentials of methanol and aqueous extracts; test fractions notably lowered immobility time in both tests; demonstrating strong antidepressant activities (p<0.001) at 400 mg/kg bodyweight dose. Through hole board test, methanolic and aqueous leaf fractions also demonstrated significant anxiolytic effects (p<0.001); the obtained outcomes for test extracts at 400 mg/kg dose were even higher than the outcomes of standard drug diazepam. Furthermore, thiopental sodium-induced sleeping time and hole cross tests were utilized to assess sedative effectiveness of test fractions at 200 and 400 mg/kg dosages. In hole cross test, after 120 minutes of drug administration, observed hole crossing values for 400 mg/kg dose of methanol and aqueous extracts were much higher than the outcome of standard drug diazepam. Additionally, in TPS induced sleeping time assay, the obtained onset and duration of sleep values (p<0.05) from the mice groups treated with 400 mg/kg dose of methanol and aqueous extracts were less significant in comparison to the outcomes (p<0.001) of standard drug diazepam. Moreover, GC-MS analysis was performed to identify probable compounds causing these actions.