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There is a growing need within the paleoclimate community for robust soil paleoproxies capable of reconstructing past terrestrial environments with high precision. Existing proxies for past mean annual air temperature (MAT), such as branched GDGTs (1) and chironomids (2), suffer from large uncertainties (i.e., ≥ 4°C error on these land temperature reconstructions), which limit their applicability.Bacteriohopanepolyols (BHPs) are pentacyclic triterpenoid membrane lipids produced by bacteria that are ubiquitous in terrestrial and aquatic environments (3). Functionalized BHPs have a large structural diversity both in the rings and head groups. They have been detected in sedimentary archives extending back 1.2 Myr (4), underscoring their considerable potential as tools for reconstructing past climatic conditions.BHPs with nucleoside (adenosyl and inosyl) head groups (Nu-BHPs) have been widely used as indicators of terrestrial organic matter input into marine systems (Rsoil) (5). Recently, a large range of previously unknown Nu-BHPs were identified thanks to a newly developed method using Ultra High Performance Liquid Chromatography – high resolution Orbitrap Mass Spectrometry (6). The relative abundances of several Nu-BHPs found in Alaskan soils were shown to correlate with pH and temperature and thus are potential paleotemperature proxies (7). To validate these correlations on a global scale, we present Nu-BHP abundances analyzed across 89 globally distributed surface soil samples. These include soils previously used to calibrate branched GDGTs (1), as well as soils from Northern Norway and Finland and Brazil, to complete coverage from the Arctic to the tropics. Complementary analyses included six soil environmental variables (pH, latitude, total organic carbon (TOC), C/N, δ¹³C, δ¹⁵N) and four climate parameters (mean annual and warmest quarter air temperature, obtained from CHELSA climatological data (8), annual and wettest quarter precipitation, retrieved from the Copernicus Climate Change Service (9)).Forty-eight Nu-BHPs were identified in soils with a pH range of 3.3-8.1 and total organic carbon (TOC) range of 0.2 and 48.4%. The most dominant compound in the dataset is adenosylhopane with 0 methylations. Of the forty-eight Nu-BHPs, thirty compounds were present in trace amounts (less than 1% of total relative abundances). The remaining eighteen Nu-BHPs were further used to investigate climatic controls on Nu-BHP abundances.This showed that only a few Nu-BHPs showed a good correlation with pH (R2 ~0.65), while temperature did not appear to influence Nu-BHP distributions. Non-metric multidimensional scaling analysis was conducted on relative abundance of these eighteen Nu-BHPs, along with the soil environmental variables and climate parameters (Fig. 1). This revealed that none of the measured parameters measured fully explains the variability in Nu-BHP distributions. We hypothesize that the main control factors instead are related to nutrient availability and/or bacterial community diversity. Future work includes investigating these variables using samples with strong nutrient and pH gradients; and known bacterial community abundances. ReferencesWeijers et al., 2007.Brooks et al., 2001. Cooke et al., 2009. Zhu et al., 2011.Talbot et al., 2014. Hopmans et al., 2021. O’Connor, 2025. Krager et al., 2017. Dorigo et al., Copernicus Climate Change Service (C3S) Climate Data Store (CDS).